Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus

Dynein axonemal light intermediate chain 1 (dnali1) is an important part of dyneins and plays role in the growth development animals. However, there little information about dnali1 fish. Herein, we cloned gene from genome olive flounder (Paralichthys olivaceus), a commercially maricultured fish China, Japan, Korea, analyzed its expression patterns different gender The DNA sequence contained 771 bp open reading frame (ORF), two sizes 5? untranslated region (5?UTR), 1499 3? (3?UTR). Two duplicated 922 nt fragments were found mRNA. first fragment downstream coding front portion 3?UTR, second was entirely located 3?UTR. Multiple alignments indicated that Dnali1 protein putative conserved coiled-coil domain. Its showed sexually dimorphic with predominant testis, lower other tissues. longer 5?UTR specifically expressed testis. highest level testis detected at stages IV V. Transient analysis repeated 3?UTR down-regulated GFP early stage zebrafish. might play especially period spermatogenesis, repetitive sequences contain some regulatory elements for cilia.